Biological Imaging Methods Ontology

A structured controlled vocabulary of sample preparation, visualization and imaging methods used in biomedical research.

Open in the Ontology Lookup Service (OLS)


3D-deconvolution [FBbi_00000357]

[3D-deconvolution; linear method]

4\',6-diamidino-2-phenylindole (DAPI) [FBbi_00000056]

[membrane impermeant probe; 4',6-diamidino-2-phenylindole (DAPI)]

5-SFX fluorescein RNA fill [FBbi_00000091]

[5-SFX fluorescein RNA fill; fluor labeled RNA fill]

7-amino-actinomycin D [FBbi_00000424]

[membrane impermeant probe; 7-amino-actinomycin D]

[14]C [FBbi_00000123]

[metabolically incorporated radioisotope; [14]C]

[32]P [FBbi_00000124]

[[32]P; metabolically incorporated radioisotope]

[33]P [FBbi_00000125]

[metabolically incorporated radioisotope; [33]P]

[35]S [FBbi_00000126]

[metabolically incorporated radioisotope; [35]S]

[3]H [FBbi_00000127]

[metabolically incorporated radioisotope; [3]H]

absorption of illumination [FBbi_00000308]

[absorption of illumination; imaged parameter]

AceFP [FBbi_00000499]

[Green fluorescent proteins from Anthozoa; AceFP]

acetone fixed tissue [FBbi_00000004]

[acetone fixed tissue; coagulating-fixative fixed tissue]

acid phosphatase [FBbi_00000158]

[acid phosphatase; enzyme label]

acridine homodimer [FBbi_00000423]

[membrane impermeant probe; acridine homodimer]

acridine orange [FBbi_00000051]

[membrane-permeant probe; acridine orange; AO]

acrolein fixed tissue [FBbi_00000005]

[acrolein fixed tissue; coagulating-fixative fixed tissue]

acrylic painted graphic [FBbi_00000236]

[acrylic painted graphic]

affinity for specific proteins [FBbi_00000459]

[affinity for specific proteins; probe for protein]

Alexa Fluor 488 [FBbi_00000440]

[fluorescent label; Alexa Fluor 488]

Alexa Fluor 546 [FBbi_00000441]

[fluorescent label; Alexa Fluor 546]

Alexa Fluor 555 [FBbi_00000443]

[Alexa Fluor 555; fluorescent label]

Alexa Fluor 568 [FBbi_00000442]

[Alexa Fluor 568; fluorescent label]

Alexa Fluor 594 [FBbi_00000444]

[fluorescent label; Alexa Fluor 594]

Alexa Fluor 610 [FBbi_00000445]

[fluorescent label; Alexa Fluor 610]

Alexa Fluor 633 [FBbi_00000446]

[Alexa Fluor 633; fluorescent label]

Alexa Fluor 647 [FBbi_00000447]

[fluorescent label; Alexa Fluor 647]

alkaline phosphatase [FBbi_00000132]

[alkaline phosphatase; enzyme label]

alpha-bungarotoxin [FBbi_00000464]

[non-immunological protein probe; alpha-bungarotoxin]

AmCyanFP [FBbi_00000487]

[Cyan fluorescent proteins from Anthozoa; AmCyanFP]

aminomethylcoumarin [FBbi_00000191]

[fluorescent label; AMCA; aminomethylcoumarin]

anaxial illumination [FBbi_00000291]

Illumination is distributed asymmetrically around the optic axis, typically achieved by a non-circular stop placed below the lower lens element and aperture of the condenser.

animation [FBbi_00000267]

A form of graphical illustration that changes with time to give a sense of motion or represent dynamic changes in the portrayal.

ANSOM [FBbi_00000349]

Aperture-less NSOM.

antibody [FBbi_00000401]

[macromolecular probe; antibody]

AQ143 [FBbi_00000491]

[Far-red fluorescent proteins from Anthozoa; AQ143]

Arachis hypogaea (PNA) [FBbi_00000428]

[Arachis hypogaea (PNA); lectin]

area detector [FBbi_00000395]

[area detector; detection method]

array scanning [FBbi_00000289]

Multiple points of illumination generated by a fixed array of sources.

array-scan confocal microscopy [FBbi_00000393]

Fixed array of sources focused to an array of spots that is swept over the specimen in raster fashion

AsRed2FP [FBbi_00000520]

[Red fluorescent proteins from Anthozoa; AsRed2FP]

atomic force microscopy [FBbi_00000259]

the scanning probe is maintained at a fixed distance above the surface e of the specimen by van der Waals forces

aurothioglucose [FBbi_00000576]

[aurothioglucose; negative staining]

avalanche photodiode (APD) [FBbi_00000297]

[avalanche photodiode (APD); spot detector]

Azami GreenFP [FBbi_00000500]

[Azami GreenFP; Green fluorescent proteins from Anthozoa]

AzuriteFP [FBbi_00000461]

[AzuriteFP; Blue fluorescent protein from Aequorea victoria]

back-scattered_electron imaging [FBbi_00000373]

imaged formed using electrons undergoing elastic scattering at very high angles, with emission of characteristic X-rays

batrachatoxin [FBbi_00000467]

[affinity for specific proteins; batrachatoxin]

beta-galactosidase [FBbi_00000077]

[lacZ, X-gal; beta-galactosidase; genetically encoded enzyme]

beta-glucuronidase [FBbi_00000078]

[beta-glucuronidase; genetically encoded enzyme; GUS, X-gluc]

biotin [FBbi_00000458]

[biotin; fluorescent label]

biotin ethylenediamine fill [FBbi_00000119]

[Neurobiotin; miscellaneous fill; biotin ethylenediamine fill]

black and white graphic [FBbi_00000225]

[computer graphic; black and white graphic]

blind deconvolution [FBbi_00000362]

[blind deconvolution; 3D-deconvolution]

Blue fluorescent protein from Aequorea victoria [FBbi_00000435]

[fluorescent protein derived from Aequorea victoria; Blue fluorescent protein from Aequorea victoria]

Blue fluorescent proteins from Anthozoa [FBbi_00000479]

[Blue fluorescent proteins from Anthozoa; fluorescent protein derived from Anthozoa]

botulinum toxin [FBbi_00000465]

[non-immunological protein probe; botulinum toxin]

boundaries between regions with different refractive index [FBbi_00000599]

[boundaries between regions with different refractive index; source of contrast]

bright-field microscopy [FBbi_00000243]

[light microscopy; bright-field microscopy; absorption of illumination; wide-field detection; makes_use_of; widefield illumination]

bsDronpa [FBbi_00000557]

[photoswitchable fluorescent protein; bsDronpa]

c-MYC peptide tag [FBbi_00000086]

[small genetically encoded tag; c-MYC peptide tag]

camera lucida assisted graphic [FBbi_00000228]

[graphic illustration; camera lucida assisted graphic]

CARS [FBbi_00000370]

coherent Raman anti-Stokes microscopy

Cascade blue [FBbi_00000192]

[fluorescent label; Cascade blue]

cationic colloidal gold [FBbi_00000053]

[optically-dense stain; cationic colloidal gold]

CeruleanFP [FBbi_00000468]

[CeruleanFP; Cyan fluorescent protein from Aequorea]

charcoal pencil graphic [FBbi_00000231]

[line art graphic; charcoal pencil graphic]

charge coupled device (CCD) [FBbi_00000294]

[area detector; charge coupled device (CCD)]

chemical composition [FBbi_00000319]

[imaged parameter; chemical composition]

chemically fixed tissue [FBbi_00000002]

[fixation method; chemically fixed tissue]

cholera toxin B [FBbi_00000431]

[cholera toxin B; lectin]

CitrineFP [FBbi_00000474]

[CitrineFP; Yellow fluorescent protein from Aequorea]

CMX rosamine (Mitotracker Red) [FBbi_00000054]

[Mitotracker X; CMX rosamine (Mitotracker Red); probe for mitochondria]

coagulating-fixative fixed tissue [FBbi_00000003]

[solvent permeabilized; coagulating-fixative fixed tissue; chemically fixed tissue]

cobalt fill [FBbi_00000120]

[cobalt fill; miscellaneous fill]

coherent illumination [FBbi_00000279]

[coherent illumination; illumination method]

color graphic [FBbi_00000226]

[computer graphic; color graphic]

colored pencil graphic [FBbi_00000232]

[line art graphic; colored pencil graphic]

compartmentalization of stain or label [FBbi_00000604]

[compartmentalization of stain or label; source of contrast]

complementary metal oxide semiconductor (CMOS) [FBbi_00000304]

[area detector; complementary metal oxide semiconductor (CMOS)]

computational method [FBbi_00000323]

[contrast-enhancing method; computational method]

computed tomography [FBbi_00001005]

Tomography in which virtual sections are taken from multiple angles and the results are processed via tomographic reconstruction software to produce 2 or 3D images.

computer graphic [FBbi_00000224]

[graphic illustration; computer graphic]

conconavalin A [FBbi_00000425]

[lectin; conconavalin A]

confocal microscopy [FBbi_00000251]

[confocal microscopy; fluorescence microscopy; fluorescence emission; narrow-field detection; makes_use_of; narrowfield illumination]

constrained iterative deconvolution [FBbi_00000360]

[3D-deconvolution; constrained iterative deconvolution]

continuous illumination [FBbi_00000283]

[continuous illumination; illumination method]

contrast-enhancing method [FBbi_00000271]

[method involved in biological imaging; contrast-enhancing method]

CopGFP [FBbi_00000501]

[CopGFP; Green fluorescent proteins from Anthozoa]

copper salt [FBbi_00000610]

[electron-dense stain; copper salt]

cresyl fast violet [FBbi_00000035]

[optically-dense stain; cresyl fast violet]

critical_point dried specimen [FBbi_00000581]

specimen from which water has been removed by a process that avoids liquid-gas and solid-gas phase transitions.

crosslinking-fixative fixed tissue [FBbi_00000008]

[crosslinking-fixative fixed tissue; chemically fixed tissue]

cryofixed tissue [FBbi_00000013]

[cryofixed tissue; fixation method]

cryostat-sectioned tissue [FBbi_00000027]

[cryostat-sectioned tissue]

Cy2 [FBbi_00000448]

[fluorescent label; Cy2]

Cy3 [FBbi_00000449]

[fluorescent label; Cy3]

Cy5 [FBbi_00000450]

[Cy5; fluorescent label]

Cyan fluorescent protein from Aequorea [FBbi_00000436]

[fluorescent protein derived from Aequorea victoria; Cyan fluorescent protein from Aequorea]

Cyan fluorescent proteins from Anthozoa [FBbi_00000480]

[Cyan fluorescent proteins from Anthozoa; fluorescent protein derived from Anthozoa]

CyPet [FBbi_00000469]

[Cyan fluorescent protein from Aequorea; CyPet]

dark-field microscopy [FBbi_00000244]

imaging with rejection of the unscattered illumination (removal of the zero order component of the diffracted wave)

darkfield illumination [FBbi_00000292]

illumination restricted to angles that exceed the acceptance angle of the image-forming lens

defocus phase contrast [FBbi_00000325]

[defocus phase contrast; optical method]

Dendra2FP [FBbi_00000549]

[photoconvertible fluorescent protein; Dendra2FP]

dEOSFP [FBbi_00000550]

[photoconvertible fluorescent protein; dEOSFP]

detection method [FBbi_00000269]

media and devices employed for recording the alterations in the illumination that result from its interaction with the sample

detection of acoustic waves [FBbi_00000376]

[detection method; detection of acoustic waves]

detection of current induced in the specimen [FBbi_00000377]

[detection method; detection of current induced in the specimen]

detection of electrons [FBbi_00000375]

[detection of electrons; detection method]

detection of photons [FBbi_00000374]

[detection method; detection of photons]

detection of UV/visible/IR photons [FBbi_00000378]

[detection of UV/visible/IR photons; detection of photons]

detection of X-ray photons [FBbi_00000379]

[detection of photons; detection of X-ray photons]

detergent permeabilized [FBbi_00000262]

Membrane permeabilization by the action of ionic or non-ionic detergents

DiA [FBbi_00000113]

[probe for lipid; DiA]

diagram [FBbi_00000033]

[portrayed image; diagram]

DiD [FBbi_00000114]

[probe for lipid; DiD]

differences in adsorption or binding of stain [FBbi_00000598]

[source of contrast; differences in adsorption or binding of stain]

differences in amount of elastic light scattering [FBbi_00000600]

[source of contrast; differences in amount of elastic light scattering]

differences in chemical composition [FBbi_00000591]

[source of contrast; differences in chemical composition]

differences in deposition of metal shadow [FBbi_00000601]

[differences in deposition of metal shadow; source of contrast]

differences in fluorescence lifetime [FBbi_00000605]

[differences in fluorescence lifetime; source of contrast]

differences in intrinsic optical density [FBbi_00000602]

[source of contrast; differences in intrinsic optical density]

differences in orientation of fluorophore [FBbi_00000606]

[differences in orientation of fluorophore; source of contrast]

differential interference contrast microscopy [FBbi_00000245]

[DIC; optical path length gradient; polarized light illumination; light microscopy; differential interference contrast microscopy; polarization-sensitive detection; Wollaston prism; Nomarski; makes_use_of]

DiI [FBbi_00000115]

[probe for lipid; DiI]

DiIC16 [FBbi_00000438]

[DiIC16; probe for lipid]

DiO [FBbi_00000116]

[probe for lipid; DiO]

DiOC6 (3,3'-dihexyloxacarbocyanine iodide) [FBbi_00000055]

[DiOC6 (3,3'-dihexyloxacarbocyanine iodide); probe for lipid]

DiR [FBbi_00000117]

[probe for lipid; DiR]

dispersed cells in vitro [FBbi_00000611]

[sample preparation method; dispersed cells in vitro]

distribution of a specific nucleic acid sequence [FBbi_00000596]

[distribution of a specific nucleic acid sequence; source of contrast]

distribution of a specific protein [FBbi_00000597]

[source of contrast; distribution of a specific protein]

distribution of epitope [FBbi_00000592]

[source of contrast; distribution of epitope]

DNA probe [FBbi_00000204]

[DNA probe; sequence-specific nucleic acid probe]

DOPI (defocussed orientation and position imaging) [FBbi_00000338]

[DOPI (defocussed orientation and position imaging); point-localization method]

Dronpa [FBbi_00000558]

[Dronpa; photoswitchable fluorescent protein]

Dronpa-3 [FBbi_00000559]

[photoswitchable fluorescent protein; Dronpa-3]

DsRed [FBbi_00000085]

[DsRed; Orange fluorescent proteins from Anthozoa]

DsRed-ES FP [FBbi_00000540]

[DsRed-ES FP; fluorescent protein timer]

DsRed-Express2FP [FBbi_00000507]

[DsRed-Express2FP; Orange fluorescent proteins from Anthozoa]

DsRed-ExpressFP [FBbi_00000506]

[DsRed-ExpressFP; Orange fluorescent proteins from Anthozoa]

DsRed-MaxFP [FBbi_00000508]

[DsRed-MaxFP; Orange fluorescent proteins from Anthozoa]

DsRed-monomerFP [FBbi_00000509]

[DsRed-monomerFP; Orange fluorescent proteins from Anthozoa]

DsRed2FP [FBbi_00000510]

[DsRed2FP; Orange fluorescent proteins from Anthozoa]

dTomatoFP [FBbi_00000511]

[dTomatoFP; Orange fluorescent proteins from Anthozoa]

E2GFP [FBbi_00000560]

[E2GFP; photoswitchable fluorescent protein]

EBFP [FBbi_00000080]

[EBFP; Blue fluorescent protein from Aequorea victoria]

ECFP [FBbi_00000081]

[Cyan fluorescent protein from Aequorea; ECFP]

EDAX imaging [FBbi_00000382]

[electron microscopy; EDAX imaging]

EELS imaging [FBbi_00000381]

[electron microscopy; ESI electron spectroscopic imaging; EELS imaging; EFTEM energy filtered TEM]

EGFP [FBbi_00000082]

[Green fluorescent protein from Aequorea; EGFP]

elastic scattering [FBbi_00000310]

[imaged parameter; elastic scattering]

elastic scattering of electrons [FBbi_00000586]

[elastic scattering of electrons]

elastic scattering of photons [FBbi_00000587]

[elastic scattering of photons]

electron bombardment CCD (EBCCD) [FBbi_00000302]

[area detector; electron bombardment CCD (EBCCD)]

electron dense label [FBbi_00000457]

[vizualization of label conjugated to probe; electron dense label]

electron density [FBbi_00000315]

[imaged parameter; electron density]

electron microscopy [FBbi_00000256]

[electron microscopy; mode of electron microscopy; microscopy with lenses; illumination by electrons; makes_use_of]

electron multiplying CCD (EMCCD) [FBbi_00000301]

[area detector; electron multiplying CCD (EMCCD)]

electron-dense stain [FBbi_00000568]

[electron-dense stain; stain with broad specificity]

elevation [FBbi_00000320]

[elevation; imaged parameter]

embedded tissue [FBbi_00000015]

[sample preparation method; embedded tissue]

EmeraldFP [FBbi_00000471]

[Green fluorescent protein from Aequorea; EmeraldFP]

emission-depletion illumination [FBbi_00000390]

[emission-depletion illumination; specimen_modifying_illumination]

enzyme label [FBbi_00000456]

[vizualization of label conjugated to probe; enzyme label]

eosin [FBbi_00000037]

Staining with an aqueous or alcoholic solution of eosin Y,colors eosinophilic structures in various shades of red, pink and orange. The eosinophilic structures are generally composed of intracellular or extracellular protein. The Lewy bodies and Mallory bodies are examples of eosinophilic structures. Most of the cytoplasm is eosinophilic. Red blood cells are stained intensely red.

eqFP611 [FBbi_00000521]

[Red fluorescent proteins from Anthozoa; eqFP611]

esterase [FBbi_00000176]

[esterase; enzyme label]

ethanol fixed tissue [FBbi_00000006]

[ethanol fixed tissue; coagulating-fixative fixed tissue]

ethidium bromide [FBbi_00000038]

[membrane impermeant probe; ethidium bromide]

ethylene glycol-bis-succinimidyl succinate fixed tissue [FBbi_00000009]

[crosslinking-fixative fixed tissue; ethylene glycol-bis-succinimidyl succinate fixed tissue; EGS]

evanescent wave microscopy [FBbi_00000617]

[evanescent wave microscopy; total internal reflection fluorescence TIRF; fluorescence microscopy]

evanescent wave scattering [FBbi_00000616]

[dark-field microscopy; evanescent wave scattering; FTIR frustrated total internal reflection]

exhaustive photon reassignment [FBbi_00000363]

[3D-deconvolution; exhaustive photon reassignment]

EYFP [FBbi_00000084]

[EYFP; Yellow fluorescent protein from Aequorea]

Far-red fluorescent proteins from Anthozoa [FBbi_00000484]

[Far-red fluorescent proteins from Anthozoa; fluorescent protein derived from Anthozoa]

farfield illumination [FBbi_00000282]

[illumination method; farfield illumination]

Fast-FT [FBbi_00000541]

[Fast-FT; fluorescent protein timer]

film [FBbi_00000303]

[area detector; film]

FIONA (fluorescence imaging with one nanometer accuracy) [FBbi_00000337]

[point-localization method; FIONA (fluorescence imaging with one nanometer accuracy)]

fixation method [FBbi_00001012]

[sample preparation method; fixation method]

FLAG peptide tag [FBbi_00000087]

[small genetically encoded tag; FLAG peptide tag]

FLIM [FBbi_00000368]

[fluorescence microscopy; FLIM]

FLIP [FBbi_00000365]

Fluorescence loss in photobleaching; a method of determining whether two populations of fluorophore are in diffusive communication with one another by observing the decrease in brightness of one population when the other population is subjected to continuous photobleaching.

fluor conjugated actin fill [FBbi_00000095]

[fluor conjugated actin fill; fluor labeled protein fill]

fluor conjugated tubulin fill [FBbi_00000109]

[fluor conjugated tubulin fill; fluor labeled protein fill]

fluor labeled protein fill [FBbi_00000094]

[intracellular filling; fluor labeled protein fill]

fluor labeled RNA fill [FBbi_00000090]

[intracellular filling; fluor labeled RNA fill]

Fluorescein (FITC) [FBbi_00000451]

[Fluorescein (FITC); fluorescent label]

fluorescence emission [FBbi_00000316]

[fluorescence emission; imaged parameter]

fluorescence microscopy [FBbi_00000246]

[UV/visible/IR illumination; light microscopy; fluorescence microscopy; fluorescence emission; makes_use_of]

fluorescence polarization (polarization angle resolved fluorescence emission) [FBbi_00000318]

[fluorescence polarization (polarization angle resolved fluorescence emission)]

fluorescence polarization microscopy [FBbi_00000603]

[fluorescence polarization microscopy; fluorescence microscopy]

fluorescent dextran fill [FBbi_00000404]

[intracellular filling; fluorescent dextran fill]

fluorescent label [FBbi_00000455]

[fluorescent label; vizualization of label conjugated to probe]

fluorescent protein derived from Aequorea victoria [FBbi_00000420]

[fluorescent protein derived from Aequorea victoria; fluorescent protein tag]

fluorescent protein derived from Anthozoa [FBbi_00000433]

[fluorescent protein tag; fluorescent protein derived from Anthozoa]

fluorescent protein derived from Arabidopsis [FBbi_00000612]

[fluorescent protein derived from Arabidopsis; fluorescent protein tag]

fluorescent protein tag [FBbi_00000405]

[fluorescent protein tag; genetically encoded tag]

fluorescent protein timer [FBbi_00000535]

[optical highlighter; fluorescent protein timer]

focussed ion beam scanning electron microscopy (FIB-SEM) [FBbi_00050000]

A method of reconstructing 3D structure by combining focussed ion beam milling to remove sucessive layers from a sample block with scanning EM to image the exposed surface between each milling step.

form birefringence [FBbi_00000593]

[source of contrast; form birefringence]

formaldehyde fixed tissue [FBbi_00000010]

[crosslinking-fixative fixed tissue; formaldehyde fixed tissue]

FRAP [FBbi_00000366]

Fluorescence loss in photobleaching; a method of determining the rate and extent of exchange between a localized population of fluorophore and a non-localized diffusible pool, by observing the rate of recovery of brightness of the localized population after photobleaching.

free hand graphic [FBbi_00000229]

[graphic illustration; free hand graphic]

freeze-substituted tissue [FBbi_00000621]

[freeze-substituted tissue; cryofixed tissue]

freeze_fracture/freeze_etch [FBbi_00000419]

[cryofixed tissue; freeze_fracture/freeze_etch]

FRET [FBbi_00000367]

Fluorescence (or Forster) resonance energy transfer; a method of estimating the distance between two fluorophores by measuring the extent of non-radiative energy transfer between the two. Experimentally, this involves selective excitation of one while observing emission from the other.

fuchsin [FBbi_00000039]

[fuchsin; optically-dense stain]

gamma ray illumination [FBbi_00001007]

[gamma ray illumination; x-ray/gamma ray illumination]

genetically encoded biotin tag [FBbi_00000418]

[small genetically encoded tag; genetically encoded biotin tag]

genetically encoded enzyme [FBbi_00000076]

[genetically encoded enzyme; genetically encoded tag]

genetically encoded tag [FBbi_00000079]

[macromolecular probe; genetically encoded tag]

giemsa [FBbi_00000040]

[giemsa; optically-dense stain]

glucose oxidase [FBbi_00000178]

[enzyme label; glucose oxidase]

glutaraldehyde fixed tissue [FBbi_00000011]

[crosslinking-fixative fixed tissue; glutaraldehyde fixed tissue]

glycerol permeabilized [FBbi_00000129]

tissue made permeable by the action of glycerol

gold [FBbi_00000202]

[electron dense label; gold]

graphic illustration [FBbi_00000223]

[graphic illustration; portrayed image]

graphite pencil graphic [FBbi_00000233]

[line art graphic; graphite pencil graphic]

Green fluorescent protein from Aequorea [FBbi_00000437]

[Green fluorescent protein from Aequorea; fluorescent protein derived from Aequorea victoria]

Green fluorescent proteins from Anthozoa [FBbi_00000481]

[Green fluorescent proteins from Anthozoa; fluorescent protein derived from Anthozoa]

grey scale graphic [FBbi_00000227]

[computer graphic; grey scale graphic]

ground state depletion scanning (GSD) [FBbi_00000618]

[ground state depletion scanning (GSD); resolution-enhancing method]

HA peptide tag [FBbi_00000034]

[small genetically encoded tag; HA peptide tag]

hand-sectioned tissue [FBbi_00000028]

[hand-sectioned tissue]

HcRed-tandemFP [FBbi_00000492]

[HcRed-tandemFP; Far-red fluorescent proteins from Anthozoa]

HcRed1FP [FBbi_00000522]

[HcRed1FP; Red fluorescent proteins from Anthozoa]

heat fixed tissue [FBbi_00000609]

[heat fixed tissue; fixation method]

Helix pomatia agglutinin [FBbi_00000429]

[Helix pomatia agglutinin; lectin]

hematoxylin [FBbi_00000041]

The staining method involves application of hemalum, which is a complex formed from aluminium ions and oxidized haematoxylin. This colors nuclei of cells (and a few other objects, such as keratohyalin granules) blue. The staining of nuclei by hemalum does not require the presence of DNA and is probably due to binding of the dye-metal complex to arginine-rich basic nucleoproteins such as histones. The mechanism is different from that of nuclear staining by basic (cationic) dyes such as thionine or toluidine blue. Staining by basic dyes is prevented by chemical or enzymatic extraction of nucleic acids. Such extractions do not prevent staining of nuclei by hemalum.

high-voltage electron microscopy (HVEM) [FBbi_00000622]

[transmission electron microscopy (TEM); high-voltage electron microscopy (HVEM)]

Hoechst 33258 [FBbi_00000417]

[membrane-permeant probe; Hoechst 33258]

Hoechst 33342 [FBbi_00000052]

[membrane-permeant probe; Hoechst 33342; HO342]

Hoffman modulation [FBbi_00000327]

[modified objective lens; Hoffman modulation]

hollow-cone illumination [FBbi_00000290]

illumination parallel or at small angles to the optical axis is attenuated or blocked by a stop

horseradish peroxidase [FBbi_00000138]

[horseradish peroxidase; enzyme label]

illumination by acoustic waves [FBbi_00000274]

[illumination by acoustic waves; illumination method]

illumination by electrons [FBbi_00000273]

[illumination method; illumination by electrons]

illumination by ions [FBbi_00000276]

[illumination method; illumination by ions]

illumination by neutrons [FBbi_00000275]

[illumination by neutrons; illumination method]

illumination by photons [FBbi_00000272]

[illumination method; illumination by photons]

illumination method [FBbi_00000268]

sources and methods for illumination

imaged parameter [FBbi_00000270]

The physical property of the specimen that leads to the modifications of the illumination that are recorded in the image.

imaging method [FBbi_00000222]

[method involved in biological imaging; imaging method]

in-focus phase contrast [FBbi_00000324]

[modified objective lens; in-focus phase contrast]

incoherent illumination [FBbi_00000280]

[incoherent illumination; illumination method]

inelastic scattering [FBbi_00000309]

[imaged parameter; inelastic scattering]

inelastic scattering of electrons [FBbi_00000588]

[inelastic scattering of electrons]

inelastic scattering of photons (Raman scattering) [FBbi_00000589]

[inelastic scattering of photons (Raman scattering)]

intensified CCD (ICCD) [FBbi_00000300]

[intensified CCD (ICCD); area detector]

intensified SIT (ISIT) [FBbi_00000299]

[area detector; intensified SIT (ISIT)]

interference contrast [FBbi_00000330]

[interference contrast; optical method]

interference reflection contrast (IRM) [FBbi_00000329]

[interference reflection contrast (IRM); optical method]

intermediate voltage electron microacopy (IVEM) [FBbi_00000623]

[transmission electron microscopy (TEM); intermediate voltage electron microacopy (IVEM)]

intracellular filling [FBbi_00000088]

visualization of the connected regions of a cell by filling with a label

intrinsic birefringence [FBbi_00000594]

[source of contrast; intrinsic birefringence]

intrinsic mass distribution [FBbi_00000607]

[intrinsic mass distribution; source of contrast]

IrisFP [FBbi_00000556]

[photoconvertible/photoswitchable fluorescent protein; IrisFP]

isolated subcellular component [FBbi_00000579]

A fragment of a cell, subcellular organelle, or macromolecular complex.

JRedFP [FBbi_00000523]

[Red fluorescent proteins from Anthozoa; JRedFP]

KaedeFP [FBbi_00000551]

[photoconvertible fluorescent protein; KaedeFP]

KatushkaFP [FBbi_00000493]

[KatushkaFP; Far-red fluorescent proteins from Anthozoa]

KFP1 [FBbi_00000561]

[photoswitchable fluorescent protein; KFP1]

Kusabira OrangeFP [FBbi_00000512]

[Kusabira OrangeFP; Orange fluorescent proteins from Anthozoa]

Kusabira OrangeFP2 [FBbi_00000513]

[Kusabira OrangeFP2; Orange fluorescent proteins from Anthozoa]

labeled primary antibody [FBbi_00000130]

[labeled primary antibody]

lead salt [FBbi_00000570]

[electron-dense stain; lead salt]

lectin [FBbi_00000402]

[macromolecular probe; lectin]

light microscopy [FBbi_00000345]

[UV/visible/IR illumination; light microscopy; microscopy with lenses; mode of light microscopy; makes_use_of]

light-sheet illumination [FBbi_00000364]

illumination in the form of a thin sheet of light directed perpendicular to the optic axis

line art graphic [FBbi_00000230]

[line art graphic; graphic illustration]

linear method [FBbi_00000354]

a computational method that is linear in the mathematical sense, meaning that image intensity is conserved and the data remain suitable for all quantitative analyses

living tissue [FBbi_00000025]

Unfixed fully hydrated tissue, typically employed to monitor dynamic changes in the living state.

lucifer yellow [FBbi_00000057]

[lucifer yellow; miscellaneous fill]

lyophilized specimen [FBbi_00000583]

specimen from which water has been removed by evaporation under vacuum at a temperature below freezing.

macromolecular probe [FBbi_00000410]

[visualization by chemical attribute; macromolecular probe]

macroscopy [FBbi_00000240]

methods for imaging objects large enough to be observed by the unaided eye

mAppleFP [FBbi_00000524]

[Red fluorescent proteins from Anthozoa; mAppleFP]

maximum likelihood deconvolution [FBbi_00000361]

[3D-deconvolution; maximum likelihood deconvolution]

mCherryFP [FBbi_00000525]

[Red fluorescent proteins from Anthozoa; mCherryFP]

mCyFP [FBbi_00000488]

[mCyFP; Cyan fluorescent proteins from Anthozoa]

Medium-FT [FBbi_00000542]

[Medium-FT; fluorescent protein timer]

membrane impermeant probe [FBbi_00000422]

[membrane impermeant probe; probes for nucleic acid]

membrane-permeant probe [FBbi_00000421]

[membrane-permeant probe; probes for nucleic acid]

mEOS2FP [FBbi_00000552]

[mEOS2FP; photoconvertible fluorescent protein]

metabolically incorporated radioisotope [FBbi_00000122]

[visualization by chemical attribute; metabolically incorporated radioisotope]

methanol fixed tissue [FBbi_00000007]

[coagulating-fixative fixed tissue; methanol fixed tissue]

method involved in biological imaging [FBbi_root_00000000]

[method involved in biological imaging]

methyl green [FBbi_00000042]

[membrane impermeant probe; methyl green]

methyl violet [FBbi_00000043]

[methyl violet; optically-dense stain]

methylene blue [FBbi_00000058]

[optically-dense stain; methylene blue]

microscopy [FBbi_00000241]

methods for forming images of objects too small to be observed with the unaided eye

microscopy with lenses [FBbi_00000343]

[microscopy with lenses]

microtome-sectioned tissue [FBbi_00000029]

[microtome-sectioned tissue]

miniSOG [FBbi_00000615]

[phototropin 2; miniSOG]

miscellaneous fill [FBbi_00000118]

[intracellular filling; miscellaneous fill]

mKate2FP [FBbi_00000495]

[mKate2FP; Far-red fluorescent proteins from Anthozoa]

mKateFP [FBbi_00000494]

[mKateFP; Far-red fluorescent proteins from Anthozoa]

mKikGR [FBbi_00000553]

[photoconvertible fluorescent protein; mKikGR]

modified objective lens [FBbi_00000353]

Uses an objective with extra components to manipulate the wave front in the plane of the objective aperture.

molybdenum compound [FBbi_00000574]

[negative staining; molybdenum compound]

montage [FBbi_00000261]

[montage; portrayed image]

mOrange2FP [FBbi_00000515]

[mOrange2FP; Orange fluorescent proteins from Anthozoa]

mOrangeFP [FBbi_00000514]

[mOrangeFP; Orange fluorescent proteins from Anthozoa]

mPlumFP [FBbi_00000496]

[mPlumFP; Far-red fluorescent proteins from Anthozoa]

mRasberryFP [FBbi_00000526]

[Red fluorescent proteins from Anthozoa; mRasberryFP]

mRFP1 [FBbi_00000527]

[Red fluorescent proteins from Anthozoa; mRFP1]

mRubyFP [FBbi_00000528]

[Red fluorescent proteins from Anthozoa; mRubyFP]

mStrawberryFP [FBbi_00000529]

[Red fluorescent proteins from Anthozoa; mStrawberryFP]

mTagBFP [FBbi_00000486]

[Blue fluorescent proteins from Anthozoa; mTagBFP]

mTFP0.7 [FBbi_00000562]

[mTFP0.7; photoswitchable fluorescent protein]

mTFP1 [FBbi_00000489]

[mTFP1; Cyan fluorescent proteins from Anthozoa]

multi-photon microscopy [FBbi_00000255]

[pulsed illumination; fluorescence microscopy; multi-photon microscopy; fluorescence emission; makes_use_of; narrowfield illumination]

multiple point scanning [FBbi_00000287]

illumination by multiple points of light simultaneously

mWasabiFP [FBbi_00000502]

[Green fluorescent proteins from Anthozoa; mWasabiFP]

narrow-field detection [FBbi_00000307]

[detection method; narrow-field detection]

narrowfield illumination [FBbi_00000278]

illumination restricted to a small portion of the field of view of the imaging lens; often one or more diffraction limited spots

nearest-neighbors deblurring [FBbi_00000359]

[non-linear method; nearest-neighbors deblurring]

nearfield illumination [FBbi_00000281]

[nearfield illumination; illumination method]

nearfield illumination [FBbi_00000619]

[makes_use_of; nearfield illumination; illumination method; evanescent wave microscopy]

nearfield illumination [FBbi_00000620]

[makes_use_of; nearfield illumination; illumination method; evanescent wave scattering]

nearfield scanning optical microscopy (ANSOM) [FBbi_00000348]

[scanning probe microscopy; nearfield scanning optical microscopy (ANSOM)]

negative staining [FBbi_00000399]

visualization by exclusion of a stain in which the specimen is immersed; compare with “positive staining.

Nile blue A [FBbi_00000059]

[optically-dense stain; Nile blue A]

Nile red [FBbi_00000060]

[probe for lipid; Nile red]

nitro blue tetrazolium chloride [FBbi_00000044]

[nitro blue tetrazolium chloride; optically-dense stain]

no-neighbors deblurring [FBbi_00000358]

[no-neighbors deblurring; non-linear method]

non-immunological protein probe [FBbi_00000463]

[non-immunological protein probe; macromolecular probe]

non-linear method [FBbi_00000355]

computational method that are mathematically non-linear; image intensity is not conserved, and the data are in general not suitable for quantitative analyses of pixel values

non-specific protein affinity [FBbi_00000460]

[non-specific protein affinity; probe for protein]

nuclear fast red [FBbi_00000045]

[optically-dense stain; nuclear fast red]

oblique illumination [FBbi_00000285]

[illumination method; oblique illumination]

OCT [FBbi_00000371]

optical coherence tomography; an interferometric method of imaging using back-scattered photons (elastic scattering)

oil painted graphic [FBbi_00000237]

[oil painted graphic]

optical highlighter [FBbi_00000434]

[optical highlighter; fluorescent protein tag]

optical method [FBbi_00000322]

[contrast-enhancing method; optical method]

optical path length gradient [FBbi_00000312]

[imaged parameter; optical path length gradient]

optically-dense stain [FBbi_00000567]

[optically-dense stain; stain with broad specificity]

Orange fluorescent proteins from Anthozoa [FBbi_00000482]

[fluorescent protein derived from Anthozoa; Orange fluorescent proteins from Anthozoa]

orcein [FBbi_00000046]

[non-specific protein affinity; orcein]

organelle-specific probe [FBbi_00000409]

[organelle-specific probe; small-molecule probe]

orientation-independent polarization microscopy [FBbi_00000351]

[orientation-independent polarization microscopy]

osmium tetroxide [FBbi_00000571]

[electron-dense stain; osmium tetroxide]

osmium tetroxide fixed tissue [FBbi_00000012]

[osmium tetroxide fixed tissue; crosslinking-fixative fixed tissue]

other negative stain [FBbi_00000577]

[other negative stain; negative staining]

other radioisotope [FBbi_00000578]

[metabolically incorporated radioisotope; other radioisotope]

PA-CFP [FBbi_00000545]

[PA-CFP; photoactivatable fluorescent protein]

PA-GFP [FBbi_00000544]

[photoactivatable fluorescent protein; PA-GFP]

PA-mCherry1FP [FBbi_00000546]

[photoactivatable fluorescent protein; PA-mCherry1FP]

PA-mRFP [FBbi_00000547]

[photoactivatable fluorescent protein; PA-mRFP]

PadronFP [FBbi_00000563]

[PadronFP; photoswitchable fluorescent protein]

PAINT (point accumulation for imaging in nanoscale topography) [FBbi_00000339]

[point-localization method; PAINT (point accumulation for imaging in nanoscale topography)]

painted graphic [FBbi_00000235]

[line art graphic; painted graphic]

PALM (photoactivation localization microscopy) [FBbi_00000335]

[PALM (photoactivation localization microscopy); makes_use_of; photo-activation illumination; point-localization method]

pastel painted graphic [FBbi_00000238]

[pastel painted graphic]

pen and ink graphic [FBbi_00000234]

[line art graphic; black and white graphic; pen and ink graphic]

peptide-nucleic acid probe [FBbi_00000432]

[sequence-specific nucleic acid probe; peptide-nucleic acid probe]

permeabilized tissue [FBbi_00000093]

Tissue treated to make cell membranes permeable, typically for the purpose of allowing access by exogenous stains or substrates.

phalloidin [FBbi_00000100]

[phalloidin; affinity for specific proteins]

Phamret [FBbi_00000548]

[photoactivatable fluorescent protein; Phamret]

phase contrast microscopy [FBbi_00000247]

[light microscopy; hollow-cone illumination; refractive index; phase contrast microscopy; makes_use_of]

Phaseolus vulgaris lectin PHA-L) [FBbi_00000427]

[Phaseolus vulgaris lectin PHA-L)]

PhiYFP [FBbi_00000531]

[PhiYFP; Yellow fluorescent proteins from Anthozoa]

photo-ablation illumination [FBbi_00000385]

[photo-ablation illumination; specimen_modifying_illumination]

photo-activation illumination [FBbi_00000387]

[specimen_modifying_illumination; photo-activation illumination]

photo-bleaching illumination [FBbi_00000384]

[photo-bleaching illumination; specimen_modifying_illumination]

photo-switching illumination [FBbi_00000386]

[photo-switching illumination; specimen_modifying_illumination]

photoactivatable fluorescent protein [FBbi_00000536]

[photoactivatable fluorescent protein; optical highlighter]

photoconvertible fluorescent protein [FBbi_00000537]

[photoconvertible fluorescent protein; optical highlighter]

photoconvertible/photoswitchable fluorescent protein [FBbi_00000538]

[photoconvertible/photoswitchable fluorescent protein; optical highlighter]

photodiode [FBbi_00000296]

[photodiode; spot detector]

photomultiplier tube (PMT) [FBbi_00000295]

[photomultiplier tube (PMT); spot detector]

photoswitchable fluorescent protein [FBbi_00000539]

[optical highlighter; photoswitchable fluorescent protein]

phototropin 2 [FBbi_00000614]

[fluorescent protein derived from Arabidopsis; phototropin 2]

piezo-electric device [FBbi_00000305]

[piezo-electric device; spot detector]

point-localization method [FBbi_00000333]

[resolution-enhancing method; point-localization method]

polarization contrast [FBbi_00000331]

[polarization contrast; optical method]

polarization microscopy [FBbi_00000248]

[polarized light illumination; light microscopy; polarization-sensitive detection; retardance; makes_use_of; polarization microscopy]

polarization-sensitive detection [FBbi_00000352]

[detection method; polarization-sensitive detection]

polarized light illumination [FBbi_00000350]

[illumination by photons; polarized light illumination]

portrayed image [FBbi_00000266]

methods and devices used for creating images by some form of artwork, as distinct from capturing images by means of some recording device

potassium permanganate [FBbi_00000572]

[electron-dense stain; potassium permanganate]

primary antibody plus labeled secondary antibody [FBbi_00000156]

[primary antibody plus labeled secondary antibody]

probe for endoplasmic reticulum [FBbi_00000412]

[organelle-specific probe; probe for endoplasmic reticulum]

probe for Golgi [FBbi_00000413]

[probe for Golgi; organelle-specific probe]

probe for lipid [FBbi_00000112]

[probe for lipid; small-molecule probe]

probe for lysosomes [FBbi_00000414]

[probe for lysosomes; organelle-specific probe]

probe for mitochondria [FBbi_00000411]

[organelle-specific probe; probe for mitochondria]

probe for protein [FBbi_00000408]

[probe for protein; small-molecule probe]

probes for nucleic acid [FBbi_00000406]

[probes for nucleic acid; small-molecule probe]

propidium iodide [FBbi_00000047]

[propidium iodide; PI; membrane impermeant probe]

pulsed illumination [FBbi_00000284]

illumination that varies with time; often achieved with pulsed lasers for the purpose of generating very-high peak illumination power

pyronine [FBbi_00000061]

[membrane-permeant probe; pyronine]

radiography [FBbi_00001000]

An imaging technique that uses high energy electromagnetic radiation, typically X-rays, as a source of illumination.

recorded image [FBbi_00000265]

methods and devices used for capturing an image of a real object; distinct from images created by artwork

Red fluorescent proteins from Anthozoa [FBbi_00000483]

[Red fluorescent proteins from Anthozoa; fluorescent protein derived from Anthozoa]

refractive index [FBbi_00000311]

[imaged parameter; refractive index]

resolution-enhancing method [FBbi_00000321]

methods for increasing the resolution beyond the classical Abbe diffraction limit

retardance [FBbi_00000313]

[retardance; imaged parameter]

Rheinberg illumination [FBbi_00000293]

illumination at angles less than the acceptance angle of the image-forming lens is attenuated by a filter to give a colored background of unscattered light; higher angle illumination may be filtered to give a contrasting color

Rhodamine [FBbi_00000452]

[fluorescent label; Rhodamine]

rhodamine 123 [FBbi_00000062]

[probe for mitochondria; rhodamine 123]

rhodamine B [FBbi_00000063]

[rhodamine B; optically-dense stain]

RNA probe [FBbi_00000215]

[RNA probe; sequence-specific nucleic acid probe]

rsCherryFP [FBbi_00000564]

[photoswitchable fluorescent protein; rsCherryFP]

rsCherryRevFP [FBbi_00000565]

[rsCherryRevFP; photoswitchable fluorescent protein]

rsFastLimeFP [FBbi_00000566]

[photoswitchable fluorescent protein; rsFastLimeFP]

sample preparation method [FBbi_00000001]

[sample preparation method; method involved in biological imaging]

saponin permeabilized [FBbi_00000264]

Saponin, a detergent-like molecule, preferentially forms holes in membranes that contain cholesterol.

saturated structured-illumination microscopy (SSIM) [FBbi_00000340]

structured illumination used at sufficiently high power to cause significant ground-state depletion of the imaged fluorophore, thus allowing for non-linear resolution enhancement

scanning electron microscopy (SEM) [FBbi_00000257]

image is formed by scanning the surface of the specimen with a beam of electrons in a raster pattern

scanning probe microscopy [FBbi_00000344]

imaging by means of a physical probe that passes over the sample

scanning tunneling microscopy [FBbi_00000347]

[scanning tunneling microscopy; scanning probe microscopy]

scanning-transmission electron microscopy [FBbi_00000380]

[scanning-transmission electron microscopy]

SCFP [FBbi_00000470]

[Cyan fluorescent protein from Aequorea; SCFP]

secondary electron generation [FBbi_00000580]

electrons generated by inelastic scattering of other radiation, the primary radiation.

secondary_electron imaging [FBbi_00000372]

image created using the electrons generated by ionization of a sample due to inelastic scattering of the primary beam of radiation

sectioned tissue [FBbi_00000026]

[sample preparation method; sectioned tissue]

sequence-specific nucleic acid probe [FBbi_00000400]

[sequence-specific nucleic acid probe; macromolecular probe]

serial block face SEM (SBFSEM) [FBbi_00000585]

A method of reconstructing 3D structure by combining serial sectioning with scanning EM.

shadowing and plating [FBbi_00000398]

visualization by depositing a label on the surface of a specimen uniformly (plating), or from a particular direction so that surface topography becomes visible (shadowing)

silicon intensified target tube (SIT) [FBbi_00000298]

[silicon intensified target tube (SIT); area detector]

silver-intensified cobalt fill [FBbi_00000121]

[silver-intensified cobalt fill]

single point scanning [FBbi_00000286]

illumination by a single spot of light that is scanned across the field of view

single sideband edge enhancement (SSBE) [FBbi_00000328]

[modified objective lens; single sideband edge enhancement (SSBE)]

single-spot confocal microscopy [FBbi_00000252]

[makes_use_of; single-spot confocal microscopy; spot detector; single point scanning]

SiriusFP [FBbi_00000462]

[SiriusFP; Blue fluorescent protein from Aequorea victoria]

slit-scan confocal microscopy [FBbi_00000392]

illumination and detection via a thin slit

slit-scanning illumination [FBbi_00000391]

illumination in the shape of a thin line that is swept over the field of view

Slow-FT [FBbi_00000543]

[Slow-FT; fluorescent protein timer]

small genetically encoded tag [FBbi_00000407]

[small genetically encoded tag]

small-molecule probe [FBbi_00000403]

[visualization by chemical attribute; small-molecule probe]

solvent permeabilized [FBbi_00000263]

permeabilization due to extraction of lipid from membranes by an organic solvent such as acetone or methanol.

source of contrast [FBbi_00000590]

The source of the variation in the imaged parameter across the field of view.

soybean agglutinin [FBbi_00000430]

[soybean agglutinin; lectin]

specimen_modifying_illumination [FBbi_00000383]

Illumination used for the purpose of inducing some observable change in the specimen during the course of an imaging session. This illumination may or may not be the same as the illumination used for imaging. Typically used in conjunction with live-cell imaging

SPIM [FBbi_00000369]

single (or selective) plane illumination.

spinning disk confocal microscopy [FBbi_00000253]

[spinning disk confocal microscopy; spinning disk scanning; narrow-field detection; charge coupled device (CCD); makes_use_of]

spinning disk scanning [FBbi_00000288]

scanning by multiple points of light arising from pinholes in a spinning disk that is illuminated by a widefield source

spot detector [FBbi_00000394]

[detection method; spot detector]

stain with broad specificity [FBbi_00000415]

[positive staining; stain with broad specificity; small-molecule probe]

stiffness [FBbi_00000314]

[stiffness; imaged parameter]

stimulated emission depletion (STED) [FBbi_00000334]

[stimulated emission depletion (STED); resolution-enhancing method; emission-depletion illumination; makes_use_of; narrowfield illumination]

STORM (stochastic optical reconstruction microscopy) [FBbi_00000336]

[makes_use_of; photo-switching illumination; point-localization method; STORM (stochastic optical reconstruction microscopy)]

stress-induced birefringence [FBbi_00000595]

[stress-induced birefringence; source of contrast]

structured illumination microscopy (SIM) [FBbi_00000332]

[structured illumination microscopy (SIM); resolution-enhancing method]

sub-nanosecond time-resolved fluorescence emission) [FBbi_00000317]

[sub-nanosecond time-resolved fluorescence emission)]

SuperfolderFP [FBbi_00000472]

[Green fluorescent protein from Aequorea; SuperfolderFP]

SYFP [FBbi_00000475]

[Yellow fluorescent protein from Aequorea; SYFP]

SYTO Blue [FBbi_00000064]

[membrane-permeant probe; SYTO Blue]

SYTO Green [FBbi_00000065]

[SYTO Green; membrane-permeant probe]

SYTO Orange [FBbi_00000066]

[SYTO Orange; membrane-permeant probe]

SYTO Red [FBbi_00000067]

[membrane-permeant probe; SYTO Red]

SYTOX Green [FBbi_00000048]

[SYTOX Green; membrane impermeant probe]

T-SapphireFP [FBbi_00000473]

[Green fluorescent protein from Aequorea; T-SapphireFP]

TagCFP [FBbi_00000490]

[Cyan fluorescent proteins from Anthozoa; TagCFP]

TagGFP [FBbi_00000503]

[TagGFP; Green fluorescent proteins from Anthozoa]

TagGFP2 [FBbi_00000504]

[Green fluorescent proteins from Anthozoa; TagGFP2]

TagRFP [FBbi_00000516]

[TagRFP; Orange fluorescent proteins from Anthozoa]

TagRFP-T [FBbi_00000517]

[TagRFP-T; Orange fluorescent proteins from Anthozoa]

TagYFP [FBbi_00000532]

[Yellow fluorescent proteins from Anthozoa; TagYFP]

taxol [FBbi_00000073]

[affinity for specific proteins; taxol]

tdKatuskaFP [FBbi_00000497]

[tdKatuskaFP; Far-red fluorescent proteins from Anthozoa]

tdRFP611 [FBbi_00000530]

[Red fluorescent proteins from Anthozoa; tdRFP611]

tdRFP639 [FBbi_00000498]

[Far-red fluorescent proteins from Anthozoa; tdRFP639]

tdTomatoFP [FBbi_00000518]

[tdTomatoFP; Orange fluorescent proteins from Anthozoa]

tetracysteine tag [FBbi_00000416]

[tetracysteine tag; small genetically encoded tag]

Tetramethyl rhodamine (TRITC) [FBbi_00000454]

fluorophore derivative of rhodamine with ex/em 547/572

tetrodotoxin [FBbi_00000466]

[affinity for specific proteins; tetrodotoxin]

TexasRed [FBbi_00000453]

fluorophore with ex/em 589/615

time lapse microscopy [FBbi_00000249]

[light microscopy; time lapse microscopy]

tissue in acrylic resin embedment [FBbi_00000017]

[tissue in acrylic resin embedment; tissue in plastic embedment]

tissue in epoxy resin embedment [FBbi_00000018]

[tissue in epoxy resin embedment; tissue in plastic embedment]

tissue in paraffin embedment [FBbi_00000020]

[tissue in paraffin embedment; tissue in wax embedment]

tissue in plastic embedment [FBbi_00000016]

[tissue in plastic embedment; embedded tissue]

tissue in polyester wax embedment [FBbi_00000021]

[tissue in polyester wax embedment; tissue in wax embedment]

tissue in polyethylene glycol embedment [FBbi_00000022]

[tissue in polyethylene glycol embedment; tissue in wax embedment]

tissue in vitreous ice embedment [FBbi_00000089]

Fully hydrated tissue rapidly frozen to produce vitrified water, and maintained continuously below the glass transition temperature of water (approximately -137 deg C

tissue in wax embedment [FBbi_00000019]

[tissue in wax embedment; embedded tissue]

toluidine blue [FBbi_00000049]

[optically-dense stain; toluidine blue]

tomography [FBbi_00001004]

An imaging technique that creates virtual sections through the use of any kind of penetrating wave.

TopazFP [FBbi_00000476]

[Yellow fluorescent protein from Aequorea; TopazFP]

transmission electron microscopy (TEM) [FBbi_00000258]

[transmission electron microscopy (TEM); makes_use_of; widefield illumination; wide-field detection]

trapping illumination [FBbi_00000389]

[specimen_modifying_illumination; trapping illumination]

tungsten compound [FBbi_00000575]

[negative staining; tungsten compound]

turboRFP [FBbi_00000519]

[turboRFP; Orange fluorescent proteins from Anthozoa]

TurboYFP [FBbi_00000533]

[Yellow fluorescent proteins from Anthozoa; TurboYFP]

two-photon laser scanning microscopy [FBbi_00000254]

[multi-photon microscopy; two-photon laser scanning microscopy]

uncaging illumination [FBbi_00000388]

[uncaging illumination; specimen_modifying_illumination]

unembedded tissue [FBbi_00000023]

[unembedded tissue; sample preparation method]

unfixed tissue [FBbi_00000014]

[unfixed tissue; fixation method]

unprocessed raw data [FBbi_00000582]

data as acquired, with no alterations that change the pixel values.

uranium compound [FBbi_00000573]

[negative staining; uranium compound]

uranyl salt [FBbi_00000569]

[uranyl salt; electron-dense stain]

UV/visible/IR illumination [FBbi_00000341]

[illumination by photons; UV/visible/IR illumination]

VenusFP [FBbi_00000477]

[Yellow fluorescent protein from Aequorea; VenusFP]

vibratome-sectioned tissue [FBbi_00000030]

[vibratome-sectioned tissue]

vidicon tube camera [FBbi_00000613]

[area detector; vidicon tube camera]

visualization by chemical attribute [FBbi_00000397]

methods of visualization that differentiate regions of the specimen on the basis of their chemical properties

visualization method [FBbi_00000031]

[visualization method; method involved in biological imaging]

visualization of contiguous regions [FBbi_00000396]

methods of visualization that differentiate between different regions or surfaces of the specimen based on contiguity

vizualization of label conjugated to probe [FBbi_00000128]

[visualization method; vizualization of label conjugated to probe]

watercolor painted graphic [FBbi_00000239]

[watercolor painted graphic]

wheat germ agglutinin [FBbi_00000426]

[lectin; wheat germ agglutinin]

whole mounted tissue [FBbi_00000024]

[whole mounted tissue; sample preparation method]

wide-field detection [FBbi_00000306]

[detection method; wide-field detection]

widefield illumination [FBbi_00000277]

illumination that covers the entire field of view of the image-forming lens

Wiener filter [FBbi_00000356]

[Wiener filter; linear method]

Wollaston prism [FBbi_00000326]

a birefringent optical element typically used to split polarized illumination into two sets of parallel but displaced rays

wtEOSFP [FBbi_00000554]

[photoconvertible fluorescent protein; wtEOSFP]

wtKIikGR [FBbi_00000555]

[wtKIikGR; photoconvertible fluorescent protein]

X-ray computed tomography [FBbi_00001002]

[X-ray tomography; CAT scan; CT scan; computed axial tomography; X-ray CT; X-ray illumination; X-ray computed tomography; makes_use_of]

X-ray fluorescence [FBbi_00000608]

[fluorescence emission; X-ray fluorescence]

X-ray illumination [FBbi_00000342]

[X-ray illumination; x-ray/gamma ray illumination]

X-ray microscopy [FBbi_00000260]

[X-ray radiography; X-ray illumination; makes_use_of; X-ray microscopy]

X-ray radiography [FBbi_00001001]

Radiography using X-rays

X-ray tomography [FBbi_00001003]

Tomography using X-ray illumination.

x-ray/gamma ray illumination [FBbi_00001006]

[illumination by photons; x-ray/gamma ray illumination]

X-Rhodamine [FBbi_00000584]

fluorophore derivative of rhodamine with ex/em 580/605

X-rhodamine RNA fill [FBbi_00000092]

[X-rhodamine RNA fill; fluor labeled RNA fill]

Yellow fluorescent protein from Aequorea [FBbi_00000439]

[fluorescent protein derived from Aequorea victoria; Yellow fluorescent protein from Aequorea]

Yellow fluorescent proteins from Anthozoa [FBbi_00000485]

[Yellow fluorescent proteins from Anthozoa; fluorescent protein derived from Anthozoa]

YO-PRO-1 [FBbi_00000068]

[membrane impermeant probe; YO-PRO-1]

yPet [FBbi_00000478]

[yPet; Yellow fluorescent protein from Aequorea]

ZsGreenFP [FBbi_00000505]

[ZsGreenFP; Green fluorescent proteins from Anthozoa]

ZsYellowFP [FBbi_00000534]

[ZsYellowFP; Yellow fluorescent proteins from Anthozoa]


Last modified December 29, 2021: Update _index.md (8db361e)