inferior ventrolateral protocerebrum local IVLP-IVLP neuron FBbt_00110125

inferior ventrolateral protocerebrum local IVLP-IVLP neuron

ID: FBbt_00110125

Neuron whose cell body is located in the ventromedial subesophageal region and innervates the ipsilateral inferior ventrolateral protocerebrum (IVLP) in both hemispheres, with pre- and postsynaptic sites found in this region. These neurons receive input from AMMC-B1b projection neurons in the IVLP. IVLP-IVLP are GABAergic neurons and are activated by an acoustic stimuli of 100 Hz, 300 Hz or pulse song stimuli (Lai et al., 2012. (Lai et al., 2012)

Auditory function was determined measuring GCaMP activation in these cells in response to an acoustic stimuli in a subset of neurons labeled with VT50245-GAL4. Presence of pre- and postsynaptic sites was determined by immunoreactivity to Dscam- and synaptotagmin-GFP localization, respectively. Connectivity between IVLP-IVLP and AMMC-B1b neurons in the IVLP and was assessed using GFP reconstitution across synaptic membranes (GRASP) in IVLP-IVLP neurons labeled with VT50245-GAL4 and AMMC-B1b neurons labeled with L5055-LexA. Neurotransmitter was determined by immunostaining with an anti-GABA antibody in neurons labeled with VT50245-GAL4 (Lai et al., 2012.