cryogenic serial block face scanning electron microscopy [FBbi_00100017]

cryogenic serial block face scanning electron microscopy

ID: FBbi_00100017

Class

Cryogenic serial block-face scanning electron microscopy is a volumetric imaging technique in which the surface of a biological specimen preserved in its near-native, vitrified state is imaged by a scanning electron microscope, then ablated by a focused ion beam (FIB-SEM) to reveal a new surface, enabling three-dimensional ultrastructural reconstruction of cells and tissues without the artifacts introduced by chemical fixation or resin embedding.

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Classification

cryogenic scanning electron microscopy
serial block face SEM (SBFSEM)

Alternative Names

Synonym	Scope	Reference
cryo FIB-SEM slice and view	exact synonym
cryo-FIB-SEM serial block face imaging	exact synonym
cryo-serial block face SEM	exact synonym
serial block face cryo-SEM	exact synonym
cryo FIB-SEM subtractive tomography	exact synonym
cryo-serial block face scanning electron microscopy	exact synonym