Split Expression

This techniques work by driving the expression of target transgenes at the intersection between the expression patterns of two hemi-driver transgenes.

Thousands of hemi-driver transgenes are now available, meaning that millions of combinations/splits are possible, each targeting some precise subset of the 10s-100s of thousands of neurons in a fly Central Nervous System (CNS). Finding the right combination for an experiment is a serious bottleneck for researchers.

FlyBase & Virtual Fly Brain (VFB) solve this problem by curating information and images recording where expression is driven by combinations of hemi-drivers.

This techniques work by driving the expression of target transgenes at the intersection between the expression patterns of two hemi-driver transgenes.This techniques work by driving the expression of target transgenes at the intersection between the expression patterns of two hemi-driver transgenes.

We used programmatic methods to generate expression statements for hemi-driver combinations with FlyLight image data hosted by VFB. Combinations are validated against a local copy of the FlyLight dataset and a structured user readable comment is generated as part of the expression statement. These hyperlink to the partner hemi-driver in FlyBase, contain the strain designation (where available) and can be automatically parsed by Virtual Fly Brain to attach expression and genetic data to a node representing the intersection.

split expression shown in Virtual Fly Brain (VFB)

Last modified December 24, 2021: adding links (66c54ea)