WED-VLP [FBbt_00110126]

WED-VLP

ID: FBbt_00110126

Neuron whose cell body is located between the optic lobe and the central brain, lateral to the antennal mechanosensory and motor center (Lai et al., 2012). It has dendritic arborization in the wedge and axonal arborization in the anterior ventrolateral protocerebrum (AVLP) (Lai et al., 2012; Baker et al., 2022). These neurons receive input in the wedge from AMMC-B1b neurons among others, and establish postsynaptic contacts in the AVLP. They are broadly tuned to respond to 100 Hz, 300 Hz, 700 Hz or pulse song stimuli (Lai et al., 2012); Baker et al., 2022). It is cholinergic (Baker et al., 2022).

Auditory function was determined measuring GCaMP activation in these cells in response to an acoustic stimuli in a subset of neurons labeled with VT34811-GAL4. Presence of pre- and postsynaptic sites was determined by immunoreactivity to Dscam- and synaptotagmin-GFP localization, respectively. Connectivity between IVLP-VLP and AMMC-B1b neurons in the IVLP was assessed using GFP reconstitution across synaptic membranes (GRASP). IVLP-IVLP neurons were labeled with VT50245-GAL4 and AMMC-B1b neurons labeled with L5055-LexA, whose expression pattern includes a subset of AMMC-B1b neurons. The fact that IVLP-VLP neurons respond to 700 Hz, whereas AMC-B1b neurons do not, indicates that IVLP-VLP neurons also receive input in the IVLP from other unidentified neurons (Lai et al., 2012). Neurotransmitter predicted based on EM data (Baker et al., 2022).