AMMC-A2 [FBbt_00110124]

AMMC-A2

ID: FBbt_00110124

Auditory projection neuron whose cell body is located on the ventrolateral side of the antennal lobe (Matsuo et al., 2016). Its cell body fiber extends medioventrally, bifurcating close to the midline and sending branches bilaterally into the inferior AMMC commissure (Lai et al., 2012; Matsuo et al., 2016). It has dendrites in AMMC zones A and B in both hemispheres and axonal terminals in the ipsilateral wedge (Lai et al., 2012; Matsuo et al., 2016). These neurons are broadly tuned to respond to 100 Hz, 300 Hz, 700 Hz or pulse song stimuli (Lai et al., 2012), with a greater response to pulse song (Baker et al., 2022). There are one or more of these cells per hemisphere (Matsuo et al., 2016) and they are cholinergic (Baker et al., 2022).

Auditory function was determined measuring GCaMP activation in these cells in response to an acoustic stimuli in a subset of neurons labeled with VT30609-GAL4. This driver also labels AMMC-B1b neurons, although these two types can be distinguished by its acoustic response: only AMMC-A2 neurons respond to 700 Hz stimuli. Presence of pre- and postsynaptic sites was determined by immunoreactivity to Dscam- and synaptotagmin-GFP localization, respectively (Lai et al., 2012). Neurotransmitter predicted from EM data (Baker et al., 2022).